AID EliSpot Assays
The Enzyme-linked immunosorbent spot (ELISPOT) is a very powerful method for monitoring immune responses in humans and animals.
The power of the EliSpot method lays in the fact that it can measure antigen-specific cytokine production on single cell level.
Most of the applications are still in the research area but there is a clear evolution towards the routine diagnostics: recent developed kits for the detection of latent TB infection and tuberculosis (TB disease) caused by Mycobacterium tuberculosis are used more and more in the routine laboratory.
Lyme disease is another example where the EliSpot method could be of use.
It makes it possible to:
- detect early the Borrelia infection
- differentiate between chronic and passed infection
- control the therapy (antibiotic treatment)
In transplantation, the major problem is the overcoming of an acute and/or chronic immune-mediated allograft rejection.
Pre- and post-transplant monitoring of cellular immunity with the Enzyme Linked ImmunoSpot (EliSpot) Assay has been shown recently in different articles to be useful in:
- predicting long term outcomes of transplant recipients
- monitoring a possible reactivation of latent CMV or EBV infection
AID has now developed the TransSpot(*) assay:
a combined tool which simultaneously allows:
- to detect latent viral infections (CMV, BK (Polyoma) and EBV)
- to predict the possible outcome of a planned transplantation
- to monitor the patient during time of immune suppression
(*) for evaluation only
Key features of the AID EliSpot Assay Kits:
- convenient, ready to use
- high sensitivity, high signal-to-noise ratio
- reproducible and reliable results
- customized kits on demand
For a complete overview of EliSpot Kits (PDF-450KB)
